Anti-Angiogenin mAbs

Cat. No.

MABL-407

Name

Anti-Angiogenin mAbs

Clone No.

AFD- 26-2F

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC, Block, ELISA, IHC

Species Reactivity

Rabbit, Human

Basic Information

Specificity

Binds to human angiogenin with a Kd of 1.6   nM. Minimal epitope is W89 and fragment 38-41 (PCKD).

Alternative Name

ANG; Ribonuclease 5; RNase 5; ALS9; HEL168;   RAA1

UniProt

P03950

Immunogen

Human angiogenin.

Application Notes

The antibody binds specifically to   angiogenin, a member of of the ribonuclease superfamily of proteins, which   interacts with actin on the surface of endothelial and smooth muscle cells,   inducing neovascularization. Due to its angiogenic activity, angiogenesis   plays a key role in the growth and invasiveness of tumour cells, and  therefore neutralising monoclonal antibodies such as 26-2F are important anticancer drugs. The monoclonal antibody 26-2F is not directly cytotoxic to tumour cells in vitro, however has been shown to have antagonistic effects in   human breast cancer xenografts in athymic mice.

Antibody First Published

Fett et al. A monoclonal antibody to human   angiogenin. Inhibition of ribonucleolytic and angionegnic activities and   localization of the antigenic epitope. Biochemistry 1994; 33(18) 5421-5427   PMID 7514035

Note on publication

Describes the generation of a   monoclonal antibody against human angiogenin and epitope mapping.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.