Key features and details | |
Cat. No. | MABL-392 |
Name | Anti-Alpha-Tubulin mAbs |
Clone No. | AFD- F2C |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human alpha-tubulin (no cross-reactivity with beta-tubulin). |
Alternative Name | TUBA; α-tubulin |
UniProt | Q71U36 |
Immunogen | The original antibody was generated via phage display from the Griffin.1 library by screening against Microtubule-Binding Proteins (MBPs) derived from HeLa S3 cells.Tubulin is the major constituent of microtubules. The alpha chain has a non-exchangeable GTP-binding site. |
Application Notes | This antibody's original format (human scFv) was successfully used in a Western blot,and its anti-myc (9E10) complexed version was successfully used for in vitro immunofluorescence staining of alpha-tubulin in HeLa cells (Nizak et al., 2003; PMID: 14617357). |
Antibody First Published | Nizak et al., Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo. Trafficking 2003; 4(11) :739-753 PMID:14617357. |
Note on publication | The original publication demonstrates the use of recombinant antibodies selected through phage display against subcellular fractions. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
