Key features and details | |
Cat. No. | MABL-391 |
Name | Anti-alpha-Toxin mAbs |
Clone No. | AFD- 3A4D10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | NTRL, WB, ELISA |
Species Reactivity | Clostridium perfringens |
Basic Information | |
Specificity | This antibody binds the alpha toxin of Clostridium perfringens. It specifically recognizes and binds the epitope 'ARGFAK' of the toxin. |
Alternative Name | PLC; Phospolipase C; Zn-metallo phospholipase C; Alpha-toxin;Hemolysin; Lecithinase; Phosphatidylcholine cholinephosphohydrolase; α-Toxin |
UniProt | Q0TV31 |
Immunogen | The orignal antibody was generated by immunizing BALB/c mice with an immunogenic toxoid prepared by incubating the alpha toxin with formaldehyde. |
Application Notes | Alpha toxin is an exotoxin, a type of bacterial haemolysin that attack blood cell membranes and cause cell rupture. Alpha toxin is an essential virulance factor in gas gangrene and causes myonecrosis in infected tissues. ELISA was used to identify the specific clones binding to alpha-toxin and epitope mapping of the antibody. 3A4D10 can even recognize denatrued toxin in a western blot assay. This antibody is capable of neutralizing the toxin in vitro and in vivo assays (PMID: 1718874). Only immunization with amino acids 247-370 of the Clostridium perfringens alpha toxin induced protection against the lethal effects of the toxin. The toxin plays a major role in pathogenesis of gas gangrene (PMID: 825650).Humanized version derived from its murine parent showed higher binding affinity (PMID: 7716162). |
Antibody First Published | Logan et al. Epitope Mapping of the Alpha-Toxin of Clostridium perfringens.Infect Immun. (1991); 59(12): 4338–4342. PMID:1718874 |
Note on publication | Describes the generation and epitope mapping of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
