Key features and details | |
Cat. No. | MABL-3046 |
Name | Anti-Tetracycline mAbs |
Clone No. | AFD- scFvTc |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds tetracycline. Tetracycline, is an antibiotic derived from microorganisms of the genus Streptomyces and are broadly used to treat bacterial infections. This antibody is reported to cross-react with aureomycin and also shows weak reactivity for oxytetracycline and doxycycline. |
Alternative Name | Tc; antibiotic; tetracycline hydrochloride; aureomycin; oxytetracycline; doxycycline |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with tetracycline hydrochloride-BSA as an immunogen. The cDNA obtained from total mRNA of the immunized mouse splenocytes was used as a template to amplify the variable domain genes and generate an scFv phage display library. Later on the antibody was selected by panning this library against tetracycline-ovalbumin conjugate. |
Application Notes | The binding characterization of this antibody for tetracycline was done using ELISA. Additionally, this antibody was also capable of cross-reacting with aureomycin, oxytetracycline and doxycycline in an ELISA. The colloidal gold labelled version of this antibody was used for the development of colloidal gold test strip for detection of tetracycline hydrochloride (CN103396484). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
