Key features and details | |
Cat. No. | MABL-2936 |
Name | Anti-Smoothelin mAbs |
Clone No. | AFD- R4A |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, WB, IHC |
Species Reactivity | canine, Chicken, Feline, Monkey, Swine, Human |
Basic Information | |
Specificity | The antibody reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells. Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. |
Alternative Name | |
UniProt | P53814 |
Immunogen | The original antibody was generated by immunizing a BALB/c mouse with a cytoskeletal extract of chicken gizzard. |
Application Notes | The antibody detected Smoothelin by western blot analysis. This antibody was used for immunohistochemical screening of different species and tissues (i.e., intestinal smooth muscle tissue of human, goat, Xenopus laevis, and chicken oesophagus, human large intestine, stomach, uterus, and prostate, smooth muscle cells) for the presence of smoothelin. COS7 cells transfected with the full-size human smoothelin cDNA showed filamentous structures that were reactive with the antibody in immunofluorescence analysis (van der Loop et al., 1996; PMID: 8707825). This antibody was used for immunohistochemistry on normal smooth muscle (Coco et al., 2009; PMID: 19950405). |
Antibody First Published | van der Loop et al. Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells J Cell Biol. 1996 Jul;134(2):401-11. PMID:8707825 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
