Key features and details | |
Cat. No. | MABL-2913 |
Name | Anti-SIRP alpha mAbs |
Clone No. | AFD- 12G6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, ELISA, FC |
Species Reactivity | Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody binds human and cynomolgus monkey SIRP alpha, which is a regulatory membrane glycoprotein and an immunoglobulin-like cell surface receptor for CD47. This protein plays a key role in intracellular signaling during synaptogenesis and in synaptic function. Cancer cells highly express CD47 that activates SIRPα and inhibit macrophage-mediated destruction. |
Alternative Name | CD172a; SIRPα; SIRPA; Signal regulatory protein α; Signal regulatory protein alpha; Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha- 1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84 |
UniProt | P78324 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with recombinant human SIRPα-hFc fusion protein. |
Application Notes | The binding reactivity of this antibody towards recombinant human SIRPα-his fusion protein was checked using ELISA. Flow cytometry was used to detect the binding of the antibody to SIRPα on the membrane of human and cynomolgus monkey SIRPα overexpressed cells. This antibody binds cynomolgus monkey SIRPα with a weaker binding affinity than human SIRPα. This antibody can effectively block the binding of CD47 protein to cell surface SIRPα (CN110734897). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
