Key features and details | |
Cat. No. | MABL-2912 |
Name | Anti-single-stranded poly(rI) RNA mAbs |
Clone No. | AFD- Jel 103 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPRIA, IF |
Species Reactivity | Species independent |
Basic Information | |
Specificity | Jel 103 is highly specific for single-stranded RNA and shows sequence specificity as it binds to poly(rI) but not to poly(dI), poly(rG) or to any other homopolymer - it does not bind randomly to calf thymus DNA. Jel 103 Fab is shown to form base stacking interactions with a Tyr residue and two salt-bridge interactions to the phosphate backbone with Lys and Arg residues. |
Alternative Name | polyriboinosinic acid; single stranded RNA; single-stranded RNA; inosine |
UniProt | |
Immunogen | Jel 103 was selected from a hybridoma cell line generated by the fusion of the MOPC 315-43 myeloma cell line with mouse spleen cells derived from C57/B1 mice which had been immunized with poly(rI)poly(dC) duplex complexed to methylated bovine serum albumin. |
Application Notes | Jel 301 can be used for the detection of single-stranded poly(rI) and for binding studies where the antibody has been used previosly in solid-phase radioimmunoassays. |
Antibody First Published | Pokkuluri et al. Preparation, characterization and crystallization of an antibody Fab fragment that recognizes RNA. Crystal structures of native Fab and three Fab-mononucleotide complexes. J Mol Biol. 1994 Oct 21;243(2):283-97. PMID:7523684 |
Note on publication | Describes the generation of jel 301 fab fragment and the structures of the fab in complex with rIP, rGDP and dIMP are reported. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
