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Anti-Shiga-toxin 2 mAbs

简要描述:

This antibody binds a conformational epitope on the A subunit of Shiga-like toxin 2 comprising region A (StxA2 residues 42 to 49), region B (StxA2 residues 96 to 100), and region C (StxA2 residues 244 to 259) (PMID: 19433543). This antibody does not bind A subunit of Stx1. StxII is a toxin produced by Shiga toxin (Stx)-producing E. coli (STEC) strains. Infections with STEC can lead to diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS).

产品参数:

Cat. No.:MABL-2900

Species:Engineer

Species Reactivity:Escherichia phage 933W (Bacteriophage 933W)

Type:Recombinate

Application:​immunotherapy, IP, neutralize, WB, ELISA

详细介绍

Key features and   details


Cat. No.

MABL-2900

Name

Anti-Shiga-toxin 2 mAbs

Clone No.

AFD-11E 10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

immunotherapy, IP, neutralize, WB, ELISA

Species Reactivity

Escherichia phage 933W (Bacteriophage 933W)

Basic Information


Specificity

This antibody binds a conformational epitope   on the A subunit of Shiga-like toxin 2 comprising region A (StxA2 residues 42   to 49), region B (StxA2 residues 96 to 100), and region C (StxA2 residues 244   to 259) (PMID: 19433543). This antibody does not bind A subunit of Stx1.   StxII is a toxin produced by Shiga toxin (Stx)-producing E. coli (STEC)   strains. Infections with STEC can lead to diarrhea, hemorrhagic colitis, and   hemolytic uremic syndrome (HUS).

Alternative Name

SLT2; stxA2; stx2A; SLT-2 A subunit; SLT-2a; SLT-IIa; Shiga-like toxin 2 subunit A; Verocytotoxin 2 subunit A; Verotoxin 2 subunit A; rRNA N-glycosidase 2

UniProt

P09385

Immunogen

The original antibody was generated by   immunizing 4 to 8 week old female BALB/c mice with 25µg of formalinized StxII   toxoid or 25µg of glutaraldehyde treated toxoid in complete Freund adjuvant.

Application Notes

The binding characterization of the original   IgG1 version of this antibody towards Shiga like toxin 2 was done using   ELISA. The original IgG1 version of this antibody was also used to   immunoprecipitate SLT-Il toxoid. This antibody is capable of neutralizing the   cytotoxicity of SLT-ll but not of SLT-I, Shiga toxin, or a variant of SLT-II   produced by E. coli that causes edema disease of swine (PMID: 3053764). This   antibody was also used a primary antibody in the detection of SLT-II in a   western blot assay. It was reported that binding of 11E10 to Stx2 prevented   the toxin from inhibiting protein synthesis in an in vitro assay but also   altered the overall cellular distribution of Stx2 in Vero cells. It was   suggested that binding of antibody 11E10 to Stx2 neutralizes the effects of   the toxin by preventing the toxin from reaching and/or inactivating the   ribosomes (PMID: 19433543).

Antibody First   Published

Perera et al. Isolation and characterization   of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic   Escherichia coli and use of the monoclonal antibodies in a colony   enzyme-linked immunosorbent assay. J Clin Microbiol. 1988 Oct;26(10):2127-31.   PMID:3053764

Note on publication

Describes the generation of 5 neutralizing   antibodies for Shiga-like toxin 2.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


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