Key features and details | |
Cat. No. | MABL-2899 |
Name | Anti-Shiga-like toxin 1 mAbs |
Clone No. | AFD- 13C4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, neutralize, WB, ELISA |
Species Reactivity | Bacteriophage H30 |
Basic Information | |
Specificity | This antibody is specific for the B subunit of Shiga-like toxin 1. This antibody does not bind B polypeptide of Stx2. Stx 1 is a toxin produced by Shiga toxin (Stx)-producing E. coli (STEC) strains. Infections with STEC can lead to diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS). |
Alternative Name | SLT1; stxB; SLT-1 B subunit; SLT-1b; SLT-Ib; Shiga-like toxin 1 subunit B; Verocytotoxin 1 subunit B; Verotoxin 1 subunit B |
UniProt | P69178 |
Immunogen | The original antibody was generated by immunization of BALB/c mice (female, 6 to 8 weeks old) with purified, biologically active Shiga-like toxin (SLT) from E. coli H30. |
Application Notes | The original IgG1 antibody neutralized the cytotoxicity of E. coli Shiga-like toxin (SLT) and were able to immunoprecipitate intact labeled toxin with Staphylococcus aureus protein A. The original IgG1 antibody neutralizes Shiga toxin from Shigella dysenteriae type 1 and Shiga-like toxic activities in crude cell extracts from Shigella flexneri, Vibrio cholerae, Vibrio parahaemolyticus, and Salmonella typhimurium. The IgG1 version of this antibody was also used in a colony blot assay for the detection of bacterial colonies which produce high levels of SLT (PMID: 3905611). This antibody neutralizes the cytotoxic and lethal activities of Stx1, This antibody was also reported to block the binding of Stx1 to its receptor, globotriaosyl ceramide by binding three nonlinear regions of the molecule that span receptor recognition sites on StxB1, one of which includes the essential residue 55N. This antibody was able to identify histidine- tagged StxB1 in a western blot assay. It did not recognize Stx1d (PMID: 17030576). |
Antibody First Published | Strockbine et al. Characterization of monoclonal antibodies against Shiga- like toxin from Escherichia coli. Infect Immun. 1985 Dec;50(3):695-700. PMID:3905611 |
Note on publication | Describes the generation and characterization of three neutralizing antibodies against Shiga-like toxin (SLT) from Escherichia coli. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
