Anti-Shiga toxin 1 mAbs

Cat. No.

MABL-2898

Name

Anti-Shiga toxin 1 mAbs

Clone No.

AFD- 5-5B

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Neutralisation, WB, ELISA

Species Reactivity

Escherichia coli

Basic Information

Specificity

This antibody is specific for the Stx1 B   subunit, and does not recognise Stx2

Alternative Name

Stx1; stx1B

UniProt

Q8X4M7

Immunogen

This antibody was raised by immunising mice   with Stx1 toxoid.

Application Notes

This antibody has been shown to bind the B subunit of Stx1 in Western blot analysis (Nakao et al, 2002). This antibody   is able to neutralise Stx1 cytotoxicity (Nakao, 2002). Flow cytometric   experiments have revealed that this antibody binds to free Stx1 B subunits,   not cell-bound Stx1, and inhibits Stx1 binding to its receptor on target   cells (Nakao et al, 2002). A Fab derived from this antibody also specifically   binds Stx1, as determined by ELISA, and neutralises Stx1 cytotoxicity (Inoue   et al, 2004).

Antibody First   Published

Nakao et al. Monoclonal Antibody to Shiga   Toxin 1, Which Blocks Receptor Binding and Neutralizes Cytotoxicity   Microbiol. Immunol., 46(11), 777―780, 2002 PMID:12516775

Note on publication

Describes the original generation of this   antibody, and its use in neutralisation assays and Western blot and FC   experiments.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.