Key features and details | |
Cat. No. | MABL-2853 |
Name | Anti-RSV F protein mAbs |
Clone No. | AFD- MEDI-493 (Palivizumab, hu129) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | NTRL, therapeutic, WB, ELISA |
Species Reactivity | RSV |
Basic Information | |
Specificity | This antibody recognizes the site A of the RSV F glycoprotein. This antibody binds the 'NSELLSLINDMPITNDQKKLMSNN' epitope (PMID: 20098425). |
Alternative Name | F2; Fusion glycoprotein F0; Protein F; F gycoprotein; Human respiratory syncytial virus; humanized mAb 129 |
UniProt | P03420 |
Immunogen | The original antibody was generated by immunizing female BALB/c mice by primary intranasal infection with A2 strain of RSV. |
Application Notes | This broadly neutralizing antibody is recommended to be used as a prophylactic agent for infants with risk for RSV infections. The binding and functional properties of this antibody were studied using ELISA and in vitro neutralization assays. The affinity of MEDI-493 was found to be 1.4 nM, equal to or slightly better than an isotype matched chimeric derivative of mAb 1129 (Johnson et al., 1997).Pretreatment of cotton rats with MEDI-493 resulted in 99% reduction of lung RSV titers at a dose of 2.5 mg/kg, corresponding to a serum concentration of 25-30 microg/mL (PMID: 9359721). MEDI-493 was safe and well-tolerated in this high risk pediatric population (PMID: 9493805). It was reported that monthly intramuscular doses of 15 mg/kg maintained mean trough serum concentrations that were above 40 microg/ml (the value associated with 99% reduction of pulmonary RSV in the cotton rat model) (PMID: 9779762). |
Antibody First Published | Johnson et al. Development of a humanized monoclonal antibody (MEDI-493) with potent in vitro and in vivo activity against respiratory syncytial virus. J Infect Dis. (1997); 176(5):1215-24. PMID:9359721 |
Note on publication | Describes the generation of humanized version of mAb 1129 against a conserved neutralizing epitope on the F glycoprotein of RSV. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
