Key features and details | |
Cat. No. | MABL-2854 |
Name | Anti-RT1-D mAbs |
Clone No. | AFD- OX-17 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Depletion, IP, WB, FC, IF |
Species Reactivity | Rat |
Basic Information | |
Specificity | The OX-17 antigenic determinant is isotypic and on the alpha chain. OX-17 labels B but not T cells from lymph node or thoracic duct lymph but very weakly labels thymocytes and does not label erythrocytes. OX-17 does not cross-react with Rat Ia-A or Mouse Ia-E antigen. The target antigen is expressed on antigen presenting cells, including B cells, macrophages and dendritic cells. RT1-D is a rat MHC class II protein. RT1-D is equivalent to mouse I-E antigen. |
Alternative Name | MHC class II RT1D alpha chain antigen; OX17; MRC OX-17; MRC OX17 |
UniProt | O98172 |
Immunogen | OX-17 was generated using BALB/c mice raised against rat spleen glycoproteins depleted of the Ia-A antigen using an OX-6 immunoaffinity column. |
Application Notes | This antibody binds to rat MHC Class I, and has been used to show that, in an in vivo rat model, antibodies against MHC class I antigens from stored blood may precipitate transfusion-related acute lung injury (Kelher, 2009). |
Antibody First Published | Fukumoto et al Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol 12:237-43 19826456222 PMID:6178598 |
Note on publication | Describes the generation and use of OX-17 to identify and purify novel Ia antigens.. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
