Key features and details | |
Cat. No. | MABL-2852 |
Name | Anti-RSPO2 mAbs |
Clone No. | AFD- h130M23-H1L2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, in vitro, in vivo, SPR |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | The antibody specifically reacts with human and mouse RSPO2. |
Alternative Name | hRspo2; R-spondin-2; Roof plate-specific spondin-2 |
UniProt | Q6UXX9 |
Immunogen | The antibody is the humanized version of the murine 130M23. |
Application Notes | The antibody was shown to completely block β-catenin signaling, demonstrating it is a specific inhibitor of RSPO2. The antibody blocked soluble RSPO2 binding to LGR5. The antibody had a binding affinity for human RSPO2 of 0.14 nM and mouse RSPO2 of 0.35 nM. The antibody demonstrated strong anti-tumor growth activity in combination with gemcitabine in a pancreatic xenograft model and inhibition of pancreatic tumor growth in vivo as a single agent and in combination with additional therapeutic agents. The humanized version of the antibody was produced. The humanized version had a binding affinity for human RSPO2 of 0.13 nM (US8802097B2). |
Antibody First Published | |
Note on publication | |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |