Anti-RSPO1 mAbs

Cat. No.

MABL-2851

Name

Anti-RSPO1 mAbs

Clone No.

AFD- 89M5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FACS, in vivo, SPR

Species Reactivity

Human, Mouse

Basic Information

Specificity

The antibody specifically binds human RSPO1.   The antibody cross-reacts with mouse RSPO1

Alternative Name

R-spondin-1; Roof plate-specific spondin-1; hRspo1

UniProt

Q2MKA7

Immunogen

The original antibody was generated by immunizing mice against recombinant human RSPO1 protein amino acids 31-263.

Application Notes

The binding affinity of the original format of the antibody to human and mouse RSPO1 was measured by surface plasmon resonance (Kd less than 0.1 nM respectively). Further, the binding affinity   of the humanized version of the antibody, h89M5-H2L2, to human RSPO1 was   measured by surface plasmon resonance (Kd less than 0.1 nM). The original   format of the antibody could inhibit tumor cell growth in vivo in an ovarian   tumor xenograft model as single agents and in combination with a   chemotherapeutic agent. The original format of the antibody could inhibit   tumor cell growth in vivo in a pancreatic tumor xenograft model in   combination with a chemotherapeutic agent. The original antibody was able to   block RSPO1-induced β-catenin signaling in HEK-293 cells. The original   antibody blocked binding of RSPO1 to LGR5 in HEK-293 cells, as measured by   FACS. The original and humanized format of the antibody, could bind to human   RSPO1 expressed on HEK-293 cells, as shown by FACS analysis (US8802097B2).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.