Key features and details | |
Cat. No. | MABL-2837 |
Name | Anti-Ricin mAbs |
Clone No. | AFD- LR12D |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Ricinus communis (Castor bean) |
Basic Information | |
Specificity | This antibody binds ricin, specifically the B chain of ricin. |
Alternative Name | rRNA N-glycosidase; Ricin B chain |
UniProt | P02879 |
Immunogen | The original antibody was isolated from a non-immune semisynthetic llama library constructed from blood samples (6 ml) collected from the left jugular vein of three research naïve mature llamas. The specific clones were selected using Luminex xMAP technology in addition to ELISA. |
Application Notes | The binding specificity of this antibody towards ricin was checked using ELISA. The original VHH version of this antibody is reported to bind ricin and ricin B chain with a dissociation constant of 12nM and 10nM respectively. This antibody can be used as a capture molecule in sandwich immunoassays. (PMID:18251258). |
Antibody First Published | Anderson et al. Multiplexed fluid array screening of phage displayed anti- ricin single domain antibodies for rapid assessment of specificity. Biotechniques. 2007 Dec;43(6):806-11. PMID:18251258 |
Note on publication | A semisynthetic llama library was constructed from non-immune llamas and binders specific for ricin toxin were selected using Luminex xMAP technology and ELISA. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
