Anti-RAP mAbs

Cat. No.

MABL-2805

Name

Anti-RAP mAbs

Clone No.

AFD- 7F1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, ELISA, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human RAP. It   does not cross-react with rabbit or murine RAP.

Alternative Name

Receptor-associated protein; low density   lipoprotein receptor-associated protein; 39-kDa receptor-associated protein

UniProt

Immunogen

This antibody was raised by immunising mice   with human placental RAP.

Application Notes

This antibody has been used to assess human   RAP expression by RAP-transfected H4 cells, derived from human neuroglioma cells, in immunostaining analysis (Kinoshita et al, 2001). It has also been   used in immunohistochemical analysis of paraffin-embedded human kidney tissue   sections (Kounnas et al, 1992), and in ELISA analysis to detect RAP binding   (Medh et al, 1995; Kounnas et al, 1993). dditionally, this antibody has been   used to immunoprecipitate RAP, and in western blot analysis of purified   B-amyloid protein and RAP mixtures (Kerr et al, 2010).

Antibody First   Published

Kounnas et al. The 39-kDa receptor-associated protein interacts with two members of the low density lipoprotein receptor family, alpha 2-macroglobulin receptor and glycoprotein 30. J Biol Chem. 1992 Oct 15;267(29):21162-6. PMID:1400426

Note on publication

Describes the original generation of this antibody, and its use in immunohistochemistry.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.