Anti-RANKL mAbs

Cat. No.

MABL-2804

Name

Anti-RANKL mAbs

Clone No.

AFD- IK22-5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

neutralising, FC, IHC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine RANKL.

Alternative Name

CD254; IK22/5; RANK Ligand; Tumor necrosis   factor ligand superfamily member 11; Osteoclast differentiation factor; ODF;   Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B   ligand; TNF-related activation-induced cytokine; TRANCE

UniProt

O35235

Immunogen

Thus antibody was raised by immunising an SD   rat with murine RANKL-transfected baby hamster kidney cells.

Application Notes

This antibody has been used in   immunohistochemical analysis of frozen Peyer's patch tissue sections   (Kobayashi et al, 2013). It has also been used in flow cytometric analysis of   RANKL cell surface expression by Th1 and Th17 cells (Nakae et al, 2007),   RANKL-transfected human embryonic kidney cells (Miyahira et al, 2003) and   murine T hybridoma 2B4.11 cells (Fionda et al, 2007). Additionally, this   RANKL-neutralising antibody has been used to assess the effects of RANKL inhibition on myoepithelial cell proliferation in mammary organoid culture   (Haslam et al, 2008). Treatment of collagen-induced arthritis (CIA) mice with   this antibody has been shown to reduce bone loss at the site of inflammation   (Kamijo et al, 2006), while administration to C57BL/6J mice induced transient   tibia and skull bone growth arrest and irreversible blockage of tooth   eruption (Lezot et al, 2015).

Antibody First   Published

Kamijo et al. Amelioration of bone loss in   collagen-induced arthritis by neutralizing anti-RANKL monoclonal antibody.   Biochem Biophys Res Commun. 2006 Aug 18;347(1):124-32. PMID14:21:16815304

Note on publication

Describes the original generation of this   antibody, and the characterisation of its effects when administered to   collagen-induced arthritis mice.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.