Anti-RAP1 mAbs

Cat. No.

MABL-2806

Name

Anti-RAP1 mAbs

Clone No.

AFD- 4C8/1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IF/ICC, IP, WB, IHC

Species Reactivity

Human, Mouse

Basic Information

Specificity

This antibody recognises human and mouse   RAP1, which is a part of the shelterin complex involved in the regulation of   telomere length and protection. Additionally, RAP1 acts as transcription   factor at extratelomeric sites regulating gene expression.

Alternative Name

TERF2IP; Telomeric repeat-binding factor   2-interacting protein 1; TERF2- interacting telomeric protein 1;   TRF2-interacting telomeric protein 1; Dopamine receptor-interacting protein   5; Repressor/activator protein 1 homolog; RAP1 homolog; hRap1; 4C8; 4C8 1

UniProt

Q9NYB0

Immunogen

This antibody was raised against human RAP1.

Application Notes

WB and IHC: RAP1 was visualised multiple   times in different Western blot analyses using RAP1 4C8/1 antibody;   furthermore, this antibody was used to identify RAP1 immunohistochemically in   invasive ductal carcinoma samples in experiments on the RAP1's role in   regulating NF-kappaB (Teo et al., 2010). WB: Tascher et al. (2017) compared   RAP1 protein amounts in mice skeletal muscle samples on Western blots using   this antibody to investigate changes in expression of telomeric proteins   during space flights. IF/ICC: RAP1 was successfully visualised in hMSCs using   RAP1 4C8/1 antibody (Zhang et al., 2019).

Antibody First   Published

Bombarde et al. TRF2/RAP1 and DNA-PK mediate   a double protection against joining at telomeric ends. EMBO J. 2010 May   5;29(9):1573-84. doi: 10.1038/emboj.2010.49. Epub 2010 Apr 20. PMID:20407424

Note on publication

This article describes the earliest published use of anti-RAP1 4C8/1 antibody in Western blot analyses in the   experiments on the mechanisms preventing end-joining in telomeric regions.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.