Key features and details | |
Cat. No. | MABL-2806 |
Name | Anti-RAP1 mAbs |
Clone No. | AFD- 4C8/1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IF/ICC, IP, WB, IHC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody recognises human and mouse RAP1, which is a part of the shelterin complex involved in the regulation of telomere length and protection. Additionally, RAP1 acts as transcription factor at extratelomeric sites regulating gene expression. |
Alternative Name | TERF2IP; Telomeric repeat-binding factor 2-interacting protein 1; TERF2- interacting telomeric protein 1; TRF2-interacting telomeric protein 1; Dopamine receptor-interacting protein 5; Repressor/activator protein 1 homolog; RAP1 homolog; hRap1; 4C8; 4C8 1 |
UniProt | Q9NYB0 |
Immunogen | This antibody was raised against human RAP1. |
Application Notes | WB and IHC: RAP1 was visualised multiple times in different Western blot analyses using RAP1 4C8/1 antibody; furthermore, this antibody was used to identify RAP1 immunohistochemically in invasive ductal carcinoma samples in experiments on the RAP1's role in regulating NF-kappaB (Teo et al., 2010). WB: Tascher et al. (2017) compared RAP1 protein amounts in mice skeletal muscle samples on Western blots using this antibody to investigate changes in expression of telomeric proteins during space flights. IF/ICC: RAP1 was successfully visualised in hMSCs using RAP1 4C8/1 antibody (Zhang et al., 2019). |
Antibody First Published | Bombarde et al. TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric ends. EMBO J. 2010 May 5;29(9):1573-84. doi: 10.1038/emboj.2010.49. Epub 2010 Apr 20. PMID:20407424 |
Note on publication | This article describes the earliest published use of anti-RAP1 4C8/1 antibody in Western blot analyses in the experiments on the mechanisms preventing end-joining in telomeric regions. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |