Key features and details | |
Cat. No. | MABL-2781 |
Name | Anti-Quadruplex DNA mAbs |
Clone No. | AFD-4E 11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IHC |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody binds to quadruplex DNA with a Kd of 2.64 nM. Binds specifically to only one type of quadruplex DNA, Oxy-2 (d(TTTTGGGG)2). |
Alternative Name | G4 DNA |
UniProt | |
Immunogen | Oxy-2 or d(TTTTGGGG)2. |
Application Notes | This antibody binds to quadruplex DNA, which is generated through the association of four guanines bound through Hoogsteen base pairing. G4-forming sequences are associated with immunoglobulin switch regions, promoter sequences, rDNA and telomeric repeats. |
Antibody First Published | Henderson et al.Detection of G-quadruplex DNA in mammalian cells.Nucleid Acids 2014; 42(2):860-869 PMID:24163102 |
Note on publication | Describes the generation of several G4-DNA-specific monoclonal antibodies and how these can be used for nuclear staining. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
