Anti-PspA mAbs

Cat. No.

MABL-2770

Name

Anti-PspA mAbs

Clone No.

AFD- 2B11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

BLI, IB, ELISA

Species Reactivity

Streptococcus pneumoniae (S. Pneumoniae)

Basic Information

Specificity

This antibody is specific for the   Streptococcus pneumoniae PspA. The epitope on PspA was defined to residues   231-242 by mutational analysis and supported by molecular docking analysis.

Alternative Name

Pneumococcal surface protein A

UniProt

A0A1M4NIX2

Immunogen

The original antibody was selected from a synthetic   human scFv library through biopanning against recombinant PspA.

Application Notes

The dissociation constant (Kd) of the   original human scFv version of this antibody was 5.3 ± 2.3 nM as measured in   a biolayer interferometry assay. This antibody detected PspA in immunoblots;   it exhibited a dose-dependent recognition of recombinant PspA with no cross-reactivity towards other tested pneumococcal antigens and also detected   endogenous PspA from pneumococcal lysates (Jang et al., 2017; PMID:27845043).

Antibody First   Published

Jang et al., Molecular characterization of a single-chain antibody variable fragment (scFv) specific for PspA from   Streptococcus pneumoniae. Biochem Biophys Res Commun. 2017 Jan   1;482(1):141-146. doi: 10.1016/j.bbrc.2016.10.150. Epub 2016 Nov 11.   PMID:27845043

Note on publication

The original publication describes the   identification and characterization of an scFv that specifically binds to   Streptococcus pneumoniae PspA.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.