Key features and details | |
Cat. No. | MABL-2764 |
Name | Anti-prothrombin mAbs |
Clone No. | AFD- POmAb:3.4.21.5; Coagulation factor II; Prothrombin Open monoclonal Antibody |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, SPR |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for the open form of prothrombin. Its epitope is located in kringle-1, comprising an extended binding interface centered at residues R90-Y93. |
Alternative Name | EC |
UniProt | P00734 |
Immunogen | |
Application Notes | The binding affinity of original format of the antibody to prothrombin was measured by surface plasmon resonance. The crystal structure of the Fab fragment of the antibody was determined. The antibody could prolong phospholipid-initiated and diluted Russell viper venom clotting time (Kumar et al., 2024; PMID: 38437497). |
Antibody First Published | Kumar et al. Cryo-EM structure and functional basis of prothrombin recognition by a Type-I anti-prothrombin antiphospholipid antibody. Blood. 2024 Feb 16:blood.2023022942. doi: 10.1182/blood.2023022942. Online ahead of print. PMID:38437497 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
