Key features and details | |
Cat. No. | MABL-2755 |
Name | Anti-Progesterone mAbs |
Clone No. | AFD- B14+ LysH31-HisH32 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody binds progesterone and also has less affinity for 5β and 5α-dihydroprogesterone. Progesterone is an endogenous steroid and progestogen sex hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. |
Alternative Name | P4; pregn-4-ene-3,20-dione; Agolutin; 17α-progesterone; Corpus luteum hormone |
UniProt | |
Immunogen | This antibody is an affinity matured version of the parent antibody P15G12C12G11 and was isolated from an scFv library generated from wild type scFv P15G12C12G11 using overlap PCR via simultaneous saturation mutagenesis at positions L94, L95, L96 (CDR3L), H50, and H58 (CDR2H). |
Application Notes | The binding specificity of this antibody towards progesterone, 5β and 5α- dihydroprogesterone and determination of percentage cross reactivity was done using ELISA. It shows an increase in discrimination against 5β-DHP and 5α-DHP, 23-fold and 15-fold, respectively, compared with the parent antibody (PMID: 15878862). |
Antibody First Published | Dubreuil et al. Fine tuning of the specificity of an anti-progesterone antibody by first and second sphere residue engineering. J Biol Chem. 2005 Jul 1;280(26):24880-7. PMID:15878862 |
Note on publication | The specificity of anti-progesterone P15G12C12G11 antibody was improved by combination of in vitro scanning saturation mutagenesis and error-prone PCR. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
