Anti-Porphyrin mAbs

Cat. No.

MABL-2725

Name

Anti-Porphyrin mAbs

Clone No.

AFD- 13G10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallisation, ELISA

Species Reactivity

Species independent

Basic Information

Specificity

13G10 was shown to bind Fe(ToCPP) using   competitive ELISA with an affinity of 20 nM. 13G10 showed that same affinity for non-metallated Fe(ToCPP). The antibody did not bind meso-   tetraphenylporphyrin, suggesting that the carboxylate groups are critical for   recognition.

Alternative Name

Fe(ToCPP);   Iron(III)-meso-alpha,alpha,alpha,beta-tetrakis- orthocarboxyphenylporphyrin

UniProt

Immunogen

BALB/c mice were immunised by injection of   iron(III)-meso-alpha,alpha,alpha,beta-tetrakis- orthocarboxyphenylporphyrin   (Fe(ToCPP)) conjugated to KLH. Splenocytes were obtained from immunised mice   and fused with the PA1 myeloma cell line to generate a hybridoma.

Application Notes

13G10 has been used in ELISAs to show   binding to various porphyrin molecules, with different metallation states and   functional groups (Quilez et al, 1996). The antibody was also shown to   exhibit catalytic activity when incubated with Fe(ToCPP) and ABTS and   hydrogen peroxide, however was later shown to also use a wider range of   hydroperoxides in catalysis (de Lauzon et al, 1999).

Antibody First   Published

Quilez et al. Artificial peroxidase-like   hemoproteins based on antibodies constructed from a specifically designed   ortho-carboxy substituted tetraarylporphyrin hapten and exhibiting a high   affinity for iron-porphyrins. FEBS Lett. 1996 Oct 14;395(1):73-6.   PMID:8849692

Note on publication

Describes the generation of 3 mAbs against a   modified porphyrin derivative (Fe(ToCPP)) and characterisation of their   binding to porphyrin molecules, as well as catalytic peroxidase activity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.