Key features and details | |
Cat. No. | MABL-2724 |
Name | Anti-PorM mAbs |
Clone No. | AFD- Nb30 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Porphyromonas gingivalis |
Basic Information | |
Specificity | This antibody binds N-terminal part comprising amino acids from 225–516 of the PorM protein of Porphyromonas gingivalis. PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. |
Alternative Name | Por secretion system protein porM/gldM; T9SS component cytoplasmic membrane protein PorM |
UniProt | B2RLE8 |
Immunogen | The original antibody was isolated from a nanobody phage-display naïve library generated from the peripheral blood of non-immunized llamas. The specific antibody was isolated by panning the library against periplasmic domain of PorM protein of Porphyromonas gingivalis. |
Application Notes | The binding specificity of this antibody towards C-terminal part of the PorM protein was determined using ELISA. The affinity of the antibody was found to be Kd= 4.5 ± 1.0 nM (PMID: 28471361). |
Antibody First Published | Duhoo et al. Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis. Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):286-293. PMID:28471361 |
Note on publication | Describes the generation of nanobodies against PorM protein of Porphyromonas gingivalis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
