Key features and details | |
Cat. No. | MABL-2713 |
Name | Anti-Polyethylene Glycol mAbs |
Clone No. | AFD- E11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC |
Species Reactivity | n/a |
Basic Information | |
Specificity | This antibody binds to the repeating subunits of the PEG backbone. The minimum PEG size that can be bound is ~ 2000 Da. Effective binding is greatly enhanced in PEG conjugates (i.e. PEG-protein, PEG- liposome, PEG-cell, PEG-nanoparticle conjugates). |
Alternative Name | PEG; PEG-derivatized molecules |
UniProt | |
Immunogen | Conjugate of an antibody and PEG-modified E. coli β-glucuronidase. |
Application Notes | This antibody can detect free PEG and PEG-modified proteins by ELISA, immunoblotting, and flow cytometry. |
Antibody First Published | Cheng TL, Cheng CM, Chen BM, Tsao DA, Chuang KH, Hsiao SW, Lin YH, Roffler SR. Monoclonal antibody-based quantitation of poly(ethylene glycol)-derivatized proteins, liposomes, and nanoparticles. Bioconjug Chem. 2005 Sep-Oct;16(5):1225-31. PMID:16173802 |
Note on publication | Describes the making of the IgG1 monoclonal antibody (E11), shows it recognised poly(ethylene glycol) (PEG) or PEG-derivatized molecules using ELISA, immunoblotting and flow cytometry. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
