Key features and details | |
Cat. No. | MABL-2705 |
Name | Anti-PLC-gamma phosphorylated mAbs |
Clone No. | AFD- AbAb1-PLCy |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody binds phosphorylated phosphoinositide phospholipase C-gamma, which mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It also plays an important role in the regulation of intracellular signaling cascades. |
Alternative Name | PLCG1; PLC1; PLC-gamma-1; PLC-γ; 1-phosphatidylinositol 4,5- bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1 |
UniProt | P19174 |
Immunogen | The original antibody was generated by immunizing rabbits with recombinant phosphorylated peptide pPLC-γ(RNPGF-pY-VEANPGGGSC) conjugated with KLH as an immunogen. |
Application Notes | The binding reactivity of this antibody to recombinant phosphorylated pPLC-γ peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of phosphorylated PLC-γ from NIH3T3 cell lysates. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pPLC-γ. The fluorescent scFv version of this antibody, when expressed intracellularly was reported to inhibit signal transduction by PLC-γ in transfected into NIH3T3 cells as observed using fluorescent microscopy (US20090233358). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
