Key features and details | |
Cat. No. | MABL-2702 |
Name | Anti-Plakophilin 1 mAbs |
Clone No. | AFD- 10B2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC, IF, IHC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody recognizes Plakophilin-1. It binds the epitope within the first 27 amino acids from the N-terminal region and it does not cross react with Plakophilin 2 and Plakophilin 3. Plakophilins influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilin-1 is thought to play a role in junctional plaques and contribute to epidermal morphogenesis. |
Alternative Name | PKP1; PKP-1; Plakophilin-1; Band 6 protein; B6P; PKP 1 |
UniProt | Q13835 |
Immunogen | This antibody was raised by immunizing mice with the N-terminus of human Plakophilin-1 protein. |
Application Notes | 10B2 is recommedned for the detection and analysis of Plakophilin-1. In order to identify potential perturbations in cell adherence, this antibody was used to detect plakophilin-1 in the skin tissue of patients with epidermolysis bullosa via immunofluorescence (Pigors et al., 2011; pmid: 21320868). Further, this antibody was demonstrated to have a potential diagnostic role in certain diseases, such as Ectodermal Dysplasia/Skin Fragility Syndrome where it was used to differentiate between a diseased and healthy skin samples (Sprecher et al., 2004; pmid: 15086548). It was also shown that 10B2 antibody can detect plakophilin-1 (PKP1) via Western blot (here: in HaCaT protein lysates) (Bonne et al., 2003; pmid: 12707304). |
Antibody First Published | Bonne et al. Defining desmosomal plakophilin-3 interactions J Cell Biol. 2003 Apr 28;161(2):403-16. doi:10.1083/jcb.200303036. Epub 2003 Apr 21. PMID:12707304 |
Note on publication | This article describes the earliest characterization and usage of this anti-plakophilin 1 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
