Anti-NC-174 mAbs

Cat. No.

MABL-2460

Name

Anti-NC-174 mAbs

Clone No.

AFD- NC6.8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization

Species Reactivity

Species independent

Basic Information

Specificity

This antibody is specific for SC45647.

Alternative Name

45412; 385425; SC45647; CP-Dpm-GA;   N-(4-Cyanophenyl)-N'- (diphenylmethyl)guanidineacetic acid;   N-(p-Cyanophenyl)-N'-(diphenylmethyl)-N''- (carboxymethyl)guanidine;   N-(P-CYANOPHENYL)-N'-DIPHENYLMETHYL-GUANIDINE-ACETIC ACID;   2-[[(benzhydrylamino)-[(4-cyanophenyl)amino]methylidene]amino]ethanoic acid;   2-{[(R)-{[4-   (aminomethyl)phenyl]amino}{[(1R)-1-phenylethyl]amino}methyl]amino}ethane-1,1-diol  

UniProt

Immunogen

This antibody was raised by immunizing a   mouse with NC-174.

Application Notes

The structure of the original antibody was   determined using x-ray crystallography (Gokulan et al., 2005; PMID:16026161,   Guddat et al,. 1994; PMID:7893280). The antibody was used for a   radioimmunoassay on N-(p-cyanophenyl)-N'-(diphenylmethyl) guanidineacetic   acid to determine the pH sensitivity of antibody binding (Viswanathan et al.,   1995; PMID:8519975).

Antibody First   Published

Guddat et al. Local and transmitted   conformational changes on complexation of an anti-sweetener Fab J Mol Biol.   1994 Feb 11;236(1):247-74. PMID:7893280

Note on publication

In the original paper crystal structures of   an Fab (NC6.8) from a murine IgG2b(kappa) antibody and its complex with a   sweet-tasting, N-,N'-,N"-trisubstituted guanidine compound (NC174) have   been determined by X-ray analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.