Key features and details | |
Cat. No. | MABL-2070 |
Name | Anti-IL-2R Beta chain mAbs |
Clone No. | AFD- Mik-beta 1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, IP, Block, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes and binds the beta chain of the IL-2 receptor and completely inhibits the IL-2 binding to the beta chain. The beta subunit is involved in receptor mediated endocytosis and transduction of the mitogenic signals of IL2. |
Alternative Name | CD122; IL-2R; IL2Rβ; IL-2RB; p70-75; p75; Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; High affinity IL-2 receptor subunit beta; Interleukin-15 receptor subunit beta; Mik-β1 |
UniProt | P14784 |
Immunogen | The original antibody was generated by immunizing BLAB/c mice with YTS cells, a human NK- like cell line that highly expresses the human IL-2R beta chain as immunogen. |
Application Notes | Mik-beta 1 inhibited binding of iodine labelled IL-2 to YTS cells in a dose dependent manner. Mik-beta 1 also successfully immunoprecipitated beta chain of the IL-2R from radio iodinated YTS extract. Biotinylated Mik-beta 1 also efficiently competes with IL-2 for binding to solubilized beta chain. Flow cytometry was used to study the cellular disctribution of beta chain, as determined by mik-beta 1 staining. This antibody completely inhibited IL-2 dependent T cell growth in presence of anti-Tac. Two-color flow cytometric analysis using anti-beta chain mAbs clearly showed that approximately 10% of peripheral blood lymphocytes manifest the beta chain and that this population is mainly composed of Leu-19+ NK cells (PMID: 2467293). Mik beta 1 antibody acts synergistically with humanized anti-Tac to prevent IL-2-induced proliferation of activated T cells (PMID: 8335891). A recombinant single chain immunotoxin of Mik-beta 1 and Pseudomonas derived exotoxin PE40 was selectively cytotoxic for cells expressing high levels of IL-2R beta (p75) subunit (PMID: 1401913). |
Antibody First Published | Tsudo et al. Characterization of the interleukin 2 receptor I8 chain using three distinct monoclonal antibodies. Proc Natl Acad Sci U S A. (1989); 86(6): 1982–1986. PMID:2467293 |
Note on publication | Describes the development and characterization of antibodies against beta chain of IL-2R. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
