Anti-IL-27p28 mAbs

Cat. No.

MABL-2069

Name

Anti-IL-27p28 mAbs

Clone No.

AFD- MM27-7B1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, therapeutic, ELISA, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for the IL-27 p28 subunit.

Alternative Name

MM27.7B1; MM27 7B1; MM277B1; Interleukin-27 subunit alpha; IL-27 subunit alpha; IL-27-A; IL27-A; p28; Il27; Il27a

UniProt

Q8K3I6

Immunogen

This antibody was raised by immunising mice with a p28–EBI3 fusion protein linked to ovalbumin polymers covered with  glutaraldehyde (OVAglu).

Application Notes

This antibody has been used to detect intracellular IL-27 in flow cytometric analysis (Bosmann et al, 2014), and to   detect serum and cell culture supernatant IL-27 in sandwich ELISA analysis   (Marillier et al, 2014). This antibody blocks IL-27, and is able to restore   IL‐9 and IL‐17A production in the presence of IL‐27 (Uyttenhove et al, 2011).   Administration of this antibody blocks graft-versus-host disease upon B6 cell   transfer to B6D2 recipient mice (Marillier et al, 2014).

Antibody First   Published

Uyttenhove et al. Amine‐reactive OVA   multimers for auto‐vaccination against cytokines and other mediators： perspectives   illustrated for GCP‐2 in L. major infection J. Leukoc. Biol.2011. 89： 1001–1007 PMID：21385949

Note on publication

Describes the original generation of this   antibody, and the characterisation of its IL- 27-inhibitory activity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.