Key features and details | |
Cat. No. | MABL-2016 |
Name | Anti-IL-10 mAbs |
Clone No. | AFD- 9D7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallisation, IP, Neutralisation, ELISA, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | The six 9D7 Fab CDR loops interact with a discontinuous IL-10M1 epitope comprised of residues 71-83 and 125-137. 9D7 non-competitively antagonises IL-10R1 binding. |
Alternative Name | Human cytokine synthesis inhibitory factor;GVHDS; IL10; Interleukin 10; Interleukin-10; IL10A; TGIF; JES3-9D7; JES3 9D7 |
UniProt | P22301 |
Immunogen | Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates. IL-10 was conjugated to JES3. The mouse non-secreting myeloma fusion partner P3X63- AG8.653, is fused to immunised rat splenocytes to produce heterohybridomas secreting rat antibody. Bioassays were used to identify neutralising mAbs, and immunochemical screens were used to identify mAbs that bind the cytokine. |
Application Notes | 9D7 has been used in ELISA experiments in order to specifically detect IL-10 and show higher circulating levels of IL-10 in patients with acute EBV-induced mononucleosis (Taga 1995). Further, 9D7 has been used to immunoprecipitate IL-10 from the supernatants of melanoma cells in order to study the production of IL-10 by melanoma cells (Qiyuan Chen 1994). A fab fragment derived from 9D7 has been used to study the noncompetitive antibody neutralisation of IL-10 by x-ray crystallography (Josephson 2002). |
Antibody First Published | Abrams et al. Strategies of Anti-Cytokine Monoclonal Antibody Development: Immunoassay of IL-10 and IL-5 in Clinical Samples Immunol Rev. 1992 Jun;127:5-24 PMID:1387110 |
Note on publication | Describes the generation of antibodies aginst cytokines. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
