Key features and details | |
Cat. No. | MABL-1946 |
Name | Anti-IgD (Igh-Ca haplotype) mAbs |
Clone No. | AFD- AMS9- 9.1.1.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, FC, IF, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for IgD (Igh-Ca haplotype). |
Alternative Name | IgD |
UniProt | |
Immunogen | This antibody was raised by immunizing C57BL/6 mice with spleen cells. |
Application Notes | PI- cells were used in an flow cytometry experiment using this antibody (Stall and Loken, 1984; pmid:6690619). A competitive binding assay on BALB/c mouse spleen cells was preformed using a radiolabelled version of this antibody (May et al, 1990; pmid:1691764). A cytotoxicity assay was preformed on mouse lymph node cells using this antibody. The antibody was 62,5 fold less toxic than ricin (May et al, 1990; pmid:1691764). BALB/c mice were given this antibody as a preventive treatment for chronic TMA- and DNCB-induced CHS (Nguyen, 2018; pmid:30218740). ELISA was preformed on TMA- specific IgM, IgG and IgE in mouse serum with this antibody (Nguyen, 2018; pmid:30218740). Fresh mouse spleen cells were used in flow cytometry using this antibody (Nguyen, 2018; pmid:30218740). B cells from mice were used for immunofluorescence with this antibody (Pereira et al, 2009; pmid:19597478). |
Antibody First Published | Stall et al. ALLOTYPIC SPECIFICITIES OF MURINE IgD AND IgM RECOGNIZED BY MONOCLONAL ANTIBODIES J Immunol. 1984 Feb;132(2) |
Note on publication | Hybridomas generated from mice immunized with allotype and H-2-incompatible spleen cells were screened by flow cytometry |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
