Anti-IFN-y mAbs

Cat. No.

MABL-1941

Name

Anti-IFN-y mAbs

Clone No.

AFD- YC2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

neutralizing, WB, ELISA

Species Reactivity

Chicken

Basic Information

Specificity

The antibody binds specifically to IFN-gamma, a dimerized soluble cytokine and the only member of the type II   class of interferon.

Alternative Name

Interferon gamma; Interferon-gamma;   IFN-gamma; IFNG; IFN-g; Type II interferon

UniProt

P49708

Immunogen

The original antibody was raised by   immunizing BALB/c mice with recombinant chicken IFN-y.

Application Notes

The specificity of this antibody (IgG2b) was   confirmed by ELISA analysis. The IFN-g ELISA was also capable of measuring   cytokine levels in the sera of chickens orally infected with Eimeria maxima.   The antibody detected the recombinant IFN-y by western blot analysis. The   antibody was capable of neutralizing IFN-g bioactivity against virus-infected   cells (Yun et al, 2000; pmid: 10713342).

Antibody First   Published

Yun et al. Chicken IFN-gamma monoclonal   antibodies and their application in enzyme‐linked immunosorbent assay. Vet I mmunol Immunopathol. 2000;73(3‐4):297‐308. PMID:10713342

Note on publication

The paper describes the generation and   characterization of the antibody, specific for chicken IFN-y for the purpose   of developing an IFN-y-specific enzyme-linked immunosorbent assay.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.