Anti-IFNα mAbs

Cat. No.

MABL-1942

Name

Anti-IFNα mAbs

Clone No.

AFD- AIFN

From

Recombinant Antibody

Isotype

Engineer antibody

Application

neutralize

Species Reactivity

Human

Basic Information

Specificity

AIFNα1bScFv01 and the AIFNα1bIgG01 whole antibody bind to IFNα1b (KD ~0.75 nM determined by SPR); the epitope consists   of the P26-N40 region in loop AB and E147-R150 region in helix E of IFNα1b -   residues L30, D32, D35 and R150 are critical for binding. The antibody also   binds to IFNR2, which shares partial epitopes with IFNα1b - binding affinity   to IFNR2 is much weaker. The antibody does not bind to recombinant IFNα2b or   IFNγ. IFNα is a cytokine and modulates the activtity of T cells, B cells and dendritic cells to regulate immunological functions, and are produced during   the early stages of the immune respose.

Alternative Name

interferon alpha; AIFNα1bScFv01; AIFNα1bIgG01; interferon α; interferon- α; IFNα1b; IFN-α1b; IFN-α; interferon   α1b; interferon-α1b; IFN-alpha; INF alpha; interferon-alpha

UniProt

P01562

Immunogen

The scFv AIFNα1bScFv01 was generated by screening a non-immune synthetic human antibody phage display library. The scFv sequence isolated was then be fused to a human IgG1 constant region to create   a whole antibody.

Application Notes

AIFNα1bIgG01, the IgG1 version of the   antibody, is able to cause downregulation of ISG15 and IFIT-1 expression ex   vivo in PBMCs, induced by either recombinant IFNα1b or naiive IFNα from SLE   patients’ sera. The antibody also reduces the total serum IgG and IgM   antibody levels in a pristane- primed lupus-like mouse model. This   neutralization occurs by out-competing IFNR2 for IFNα1b, which prevents the   formation of the IFNAR2-IFNα interaction and the IFNα-mediated signaling   pathway.

Antibody First   Published

Ouyang et l. Structural insights into a   human anti-IFN antibody exerting therapeutic potential for systemic lupus   erythematosus J Mol Med (Berl). 2012 Jul;90(7):837-46 PMID:22307521

Note on publication

Describes the generation of anti-IFNα   antibody by non-immune human phage display. The antibody was characterized   both in vivo and in vitro, and the crystal structure of the antibody scFv   with IFNα1b was solved.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.