Key features and details | |
Cat. No. | MABL-1942 |
Name | Anti-IFNα mAbs |
Clone No. | AFD- AIFN |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize |
Species Reactivity | Human |
Basic Information | |
Specificity | AIFNα1bScFv01 and the AIFNα1bIgG01 whole antibody bind to IFNα1b (KD ~0.75 nM determined by SPR); the epitope consists of the P26-N40 region in loop AB and E147-R150 region in helix E of IFNα1b - residues L30, D32, D35 and R150 are critical for binding. The antibody also binds to IFNR2, which shares partial epitopes with IFNα1b - binding affinity to IFNR2 is much weaker. The antibody does not bind to recombinant IFNα2b or IFNγ. IFNα is a cytokine and modulates the activtity of T cells, B cells and dendritic cells to regulate immunological functions, and are produced during the early stages of the immune respose. |
Alternative Name | interferon alpha; AIFNα1bScFv01; AIFNα1bIgG01; interferon α; interferon- α; IFNα1b; IFN-α1b; IFN-α; interferon α1b; interferon-α1b; IFN-alpha; INF alpha; interferon-alpha |
UniProt | P01562 |
Immunogen | The scFv AIFNα1bScFv01 was generated by screening a non-immune synthetic human antibody phage display library. The scFv sequence isolated was then be fused to a human IgG1 constant region to create a whole antibody. |
Application Notes | AIFNα1bIgG01, the IgG1 version of the antibody, is able to cause downregulation of ISG15 and IFIT-1 expression ex vivo in PBMCs, induced by either recombinant IFNα1b or naiive IFNα from SLE patients’ sera. The antibody also reduces the total serum IgG and IgM antibody levels in a pristane- primed lupus-like mouse model. This neutralization occurs by out-competing IFNR2 for IFNα1b, which prevents the formation of the IFNAR2-IFNα interaction and the IFNα-mediated signaling pathway. |
Antibody First Published | Ouyang et l. Structural insights into a human anti-IFN antibody exerting therapeutic potential for systemic lupus erythematosus J Mol Med (Berl). 2012 Jul;90(7):837-46 PMID:22307521 |
Note on publication | Describes the generation of anti-IFNα antibody by non-immune human phage display. The antibody was characterized both in vivo and in vitro, and the crystal structure of the antibody scFv with IFNα1b was solved. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
