Key features and details | |
Cat. No. | MABL-1940 |
Name | Anti-IFN-y mAbs |
Clone No. | AFD- YC1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralizing, WB, ELISA |
Species Reactivity | Chicken |
Basic Information | |
Specificity | The antibody binds specifically to IFN-gamma, a dimerized soluble cytokine and the only member of the type II class of interferon. |
Alternative Name | Interferon gamma; Interferon-gamma; IFN-gamma; IFNG; IFN-g; Type II interferon |
UniProt | P49708 |
Immunogen | The original antibody was raised by immunizing BALB/c mice with recombinant chicken IFN-y. |
Application Notes | The specificity of this antibody (IgG2b) was confirmed by ELISA analysis. The IFN-g ELISA was also capable of measuring cytokine levels in the sera of chickens orally infected with Eimeria maxima. The antibody detected the recombinant IFN-y by western blot analysis. The antibody was capable of neutralizing IFN-g bioactivity against virus-infected cells (Yun et al., 2000; pmid: 10713342). |
Antibody First Published | Yun et al. Chicken IFN-gamma monoclonal antibodies and their application in enzyme‐linked immunosorbent assay. Vet I mmunol Immunopathol. 2000;73(3‐4):297‐308. PMID:10713342 |
Note on publication | The paper describes the generation and characterization of the antibody, specific for chicken IFN-y for the purpose of developing an IFN-y-specific enzyme-linked immunosorbent assay. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
