Anti-Human Cytokeratin 8 mAbs

Cat. No.

MABL-1902

Name

Anti-Human Cytokeratin 8 mAbs

Clone No.

AFD- 1E8-C6-B4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IP, IF

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for the C-terminus   of cytokeratin 8, one member of a diverse group of proteins called   intermediate filament proteins. These proteins are often expressed in pairs.   Cytokeratin 8 pairs with Cytokeratin 18 to form a component of the   cytoskeleton in simple epithelial cells. Cytokeratin 8 is present on the   surface of intact hepatocytes, hepatocellular carcinoma cells, and breast   cancer cells.

Alternative Name

K8; Cytokeratin 8; CK 8; 1E8; Keratin, type   II cytoskeletal 8; Keratin-8; Type-II keratin Kb8; 1E8

UniProt

P05787

Immunogen

The original antibody was generated by   immunizing mice against the C-terminus (amino acids 472-483) of human   Cytokeratin 8.

Application Notes

The original antibody detected CK 8 by   western blot from BT20 breast cancer cells. Immunofluorescence was performed  on BT20 cells using this antibody, showing the carboxyl terminus of CK 8 was   exposed on the surfaces of breast cancer cells. The Fab fragment of the   antibody was used for inhibition experiments on breast cancer cell lines   BT20, MDA-MB-157, MCF-7 and purified CK 8. Results showed a decreased   plasminogen binding in all the experiments. The Fab fragment was used to   study the role of cell-surface CK 8 in promoting the activation of   plasminogen by t-PA in MCF-7 cells; preincubation of MCF-7 cells with the Fab   fragment caused a concentration-dependent decrease in the ability of the cell   cultures to promote plasminogen activation (Hembrough et al, 1996;   pmid:8810346).

Antibody First   Published

Hembrough et al. Cell-surface cytokeratin 8   is the major plasminogen receptor on breast cancer cells and is required for   the accelerated activation of cell-associated plasminogen by tissue-type   plasminogen activator J Biol Chem. 1996 Oct 11;271(41):25684-91. PMID:8810346

Note on publication

The paper describes the generation and   characterization of an antibody against cytokeratin 8 for the purpose of   defining its immunological, cellular, and biochemical characteristics.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.