Key features and details | |
Cat. No. | MABL-1818 |
Name | Anti-Her2/neu mAbs |
Clone No. | AFD- HerG1M |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, in vivo, inhibit, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human Her2/neu. |
Alternative Name | CD340; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2 |
UniProt | P04626 |
Immunogen | The original antibody was selected from a humanized VH single domain antibody gene library by screening against Her2/neu antigen. |
Application Notes | The binding specificity of this humanized single-domain antibody (sdAb; VHH) was tested in an ELISA. This antibody was used to generate an anti-Her2/neu and anti-human Ig bi-specific construct together with Ab04651 [C3]. The bi-specific construct was tested in vitro on BT474 breast cancer cells using a CCK8 assay and demonstrated a significant inhibitory effect on the growth of the breast cancer cells (approximately 50 times more effective than Herceptin). The bi-specific construct also exhibited a significantly prolonged stability in vivo in mice compared to the sdAb version of the antibody (CN110964113B). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
