Key features and details | |
Cat. No. | MABL-1817 |
Name | Anti-HER2 mAbs |
Clone No. | AFD- FRP5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | agonist, IP, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes an extracellular epitope of human HER2. |
Alternative Name | CD340; NEU; HER-2; HER-2/neu; ERBB2; MLN 19; NGL; TKR1; erb-b2 receptor tyrosine kinase 2; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2 |
UniProt | P04626 |
Immunogen | The original antibody was generated by immunizing female BALB/c mice with intact SKBR3 human breast tumor cells. |
Application Notes | This antibody has been successfully used to immunoprecipitate p185 erbB-2 protein from human SKBR3 breast tumor cell lysates and human erbB-2-transfected mouse HCll R111 cells. Furthermore, the binding of this antibody to surface-expressed HER2 on SKBR3 cells was determined using flow cytometry. Treatment with this antibody led to a rapid increase in the phosphotyrosine content of the erbB-2 protein in various cell lines. The binding of this antibody stimulated the turnover of the erbB-2 protein but did not stimulate DNA synthesis in HCll R111 cells. Therefore, it can be inferred that this antibody acts as a partial ligand agonist (Harwerth et al., 1992; PMID： 1353079). The binding affinity of this antibody for human HER2 was determined using ELISA (Wels et al., 1992; PMID： 1369487). The scFv version of this antibody was used in the generation of scFv(FRP5)-ETA, an antibody-exotoxin recombinant protein and tested for its anti-tumor activity (Harwerth et al., 1993; PMID：7903153). |
Antibody First Published | Harwerth et al. Monoclonal antibodies against the extracellular domain of the erbB-2 receptor function as partial ligand agonists. J Biol Chem. 1992 Jul 25;267(21)：15160-7. PMID：1353079 |
Note on publication | The original artucle describes the generation and characterization of four antibodies against extracellular domain of human HER2. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
