Anti-GD3 mAbs

Cat. No.

MABL-1664

Name

Anti-GD3 mAbs

Clone No.

AFD- 3D2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IHC

Species Reactivity

Human

Basic Information

Specificity

The original antibody is specific for GD3, a tumor-associated antigens.

Alternative Name

disialogangliosides GD3

UniProt

Immunogen

The original antibody was originated by immunizing   mice with multi-antigen peptides GD3P4.

Application Notes

The original format of the antibody was   assessed by immunostaining on a thin-layer chromatography plate (ITLC) using   a ganglioside fraction purified from human melanoma tumors. It bound specifically   to GD3 and not to any other tested ganglioside. The binding specificity of the original format of the   antibody was also investigated using bovine brain ganglioside fraction, where   it slightly bound to GT1b. The original format of the antibody bound to the   human melanoma SK MEL-28 cells (approximately 14% of the cells were   recognized). The original format of the antibody was used for immunostaining of tumor tissue (Popa et al., 2006; PMID: 16458892).

Antibody First   Published

Popa et al. GD3-replica peptides selected from a phage peptide library induce a GD3 ganglioside antibody response. FEBS Lett. 2006 Feb 20;580(5)：1398-404. doi： 10.1016/j.febslet.2006.01.063. Epub 2006 Jan 30.   PMID：16458892

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.