Key features and details | |
Cat. No. | MABL-1665 |
Name | Anti-GD3 mAbs |
Clone No. | AFD- Ha1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IHC |
Species Reactivity | Rat, Mouse |
Basic Information | |
Specificity | The original antibody binds to the disialylated gangliosides GD1b, GD3, GT1b, and GQ1b and weakly to GM3 and GD1a. It does not bind to structurally dissimilar glycolipids, including GM1, GM2, globoside, and sulphatide. |
Alternative Name | disialylated gangliosides; GD1b; GT1b; GQ1b; GD3 |
UniProt | |
Immunogen | The original antibody was generated from peripheral blood B cells of an affected patient. |
Application Notes | The original format of the antibody bound to a wide array of structures in the rodent peripheral nervous system, including dorsal root ganglia, peripheral nerve axonal profiles, compact myelin, nodes of Ranvier, motor end plates, and muscle spindles. In both rat and mouse dorsal root ganglia, the original format of the antibody stained the plasma membrane and cytoplasm of neurons. The antibody staining extended into the axonal lumen of myelinated nerve fibers. In teased fibers from mouse sciatic nerve, antibody-labeled compact myelin exposed at points of damage, created during the teasing process, and labeled axons protruding beyond ruptured myelin sheaths. It also labeled nodes of Ranvier in some intact teased fibers. In both mouse and rat, the original format of the antibody strongly labeled capillaries within the nerve bundle. In the rat muscle spindle, the antibody bound strongly to a region immediately adjacent to the intrafusal fibers, corresponding to the area defined by the internal capsule. |
Antibody First Published | Willison et al. A Somatically Mutated Human Antiganglioside IgM Antibody that Induces Experimental Neuropathy in Mice Is Encoded by the Variable Region Heavy Chain Gene, V1-18. J Clin Invest. 1996 Mar 1;97(5)：1155-64. doi： 10.1172/JCI118529. PMID：8636426 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
