Key features and details | |
Cat. No. | MABL-1663 |
Name | Anti-GD2 mAbs |
Clone No. | AFD- KM666 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assays, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the human disialoganglioside GD2, a surface antigen highly expressed on human neuroblastoma cells. |
Alternative Name | KM1138; KM8138 |
UniProt | Q9UI17 |
Immunogen | The original antibody was generated by immunizing Balb/c mic with human-neuroblastoma- cell-line-IMR32-derived GD2. |
Application Notes | The specificity of the original antibody (mouse IgG3) was tested via ELISA, showing strong binding activity to GD2 ganglioside. The antibody exhibited significant reactivity against neuroblastoma, brain tumor, and melanoma cell lines, as well as notable complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC) in vitro. A chimeric version of this antibody, KM1138, was created by combining the mouse variable regions with human constant regions. Compared to the original antibody, the chimeric antibody displayed equivalent binding to GD2 and similar CDC activity but demonstrated superior ADCC activity. A humanized version of this antibody, KM8138, was created and tested for its ability to retain binding specificity and cytotoxic activity. The humanized antibody maintained a strong affinity to GD2 ganglioside, exhibited comparable CDC to the original and chimeric antibodies, and surpassed its predecessors in terms of ADCC. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
