Anti-G mAbs

Cat. No.

MABL-1639

Name

Anti-G mAbs

Clone No.

AFD- HENV-26

From

Recombinant Antibody

Isotype

Engineer antibody

Application

flow cytometry, in vivo, neutralization, SPR, X-ray crystallography, ELISA

Species Reactivity

Hendra henipavirus, Nipah virus

Basic Information

Specificity

The antibody is specific for RBP of HeV and   NiV. The antibody epitope overlaps with the receptor binding sites of RBP.

Alternative Name

RBP; Glycoprotein; Glycoprotein G;   Attachment glycoprotein; Receptor binding protein;

UniProt

Q9IH62; F4YH71; H6V875; O89343

Immunogen

The original antibody was isolated from an   immune human individual.

Application Notes

The specificity of the original format of   the antibody for recombinant RBP head domain proteins from HeV, NiVM, or NiVB   was confirmed by ELISA analysis (EC50 = 0.14,0.09 and 0.07 ug/mL   respectively). The antibody could neutralize HeV, NiVB and NiVM in In vitro  neutralization assays (IC50= 0.07, 0.03 and 0.04 ug/mL respectively). The   antibody could bind to the full-length RBPs by flow cytometry. The EC50   values for binding of the antibody ranged from 325 to 343 ng/mL for binding   to HeV, NiVM, or NiVB. The kinetics of binding of the antibody to RBPs was   measured on a biosensor to determine affinity (KD= 2.9, 2.2, or 1.0 nM for   HeV, NiVM, or NiVB, respectively). The crystal structures of Fab fragment of   the antibody in complex with both HeV-RBP and NiV-RBP were determined. The   antibody protected ferrets in lethal models of infection with NiV (Dong et   al., 2020; PMID： 33306954).

Antibody First   Published

Dong et al. Potent Henipavirus   Neutralization by Antibodies Recognizing Diverse Sites on Hendra and Nipah   Virus Receptor Binding Protein Cell. 2020 Dec 10;183(6):1536-1550.e17. doi:   10.1016/j.cell.2020.11.023. PMID:33306954

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.