Key features and details | |
Cat. No. | MABL-1640 |
Name | Anti-GA24/19 mAbs |
Clone No. | AFD- A24 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA, transgenic studies |
Species Reactivity | Tobacco |
Basic Information | |
Specificity | The anti-GA24/19 monoclonal antibody showed specific reactivity to GA24, GA19 and GA15. These are precursors to bioactive gibberellins. |
Alternative Name | |
UniProt | |
Immunogen | An scFv was prepared by generating a hybridoma cell line which produced an anti GA24/19 monoclonal antibody, from which the antibody genes were cloned to construct an anti-GA14/19 scFv gene. |
Application Notes | RIA demonstrated the specific binding of A24 to GA24. The sequence was cloned into expression cassettes for expression in tobacco plants; these plants showed reduced growth (shorter stature) (Shimada et al, 1999). |
Antibody First Published | Shimada et al, 1999. Expression of a functional single-chain antibody against GA24/19 in transgenic tobacco. Biosci Biotechnol Biochem. 1999 Apr;63(4):779-83. |
Note on publication | Describes the original generation of A24 and its application to transgenic plant studies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
