Key features and details | |
Cat. No. | MABL-1630 |
Name | Anti-Ft OAg mAbs |
Clone No. | AFD- N62 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, WB |
Species Reactivity | Francisella tularensis |
Basic Information | |
Specificity | This antibody binds the non-reducing end of the O-antigen (OAg) of the gram-negative bacterium Francisella tularensis (Ft). The antibody N62 binds mainly to the terminal Qui4NFm sugar residue at the nonreducing end of OAg. |
Alternative Name | O-antigen of Francisella tularensis; O-antigen ligase family protein; non- reducing end of F. tularensis OAg |
UniProt | A0A0B6E216 |
Immunogen | The original antibody was generated by immunizing BALB/cJ mice intradermally with Francisella tularensis holarctica strain LVS (live vaccine strain) and later on a booster immunization with an outer membrane-enriched LVS preparation of F. tularensis. |
Application Notes | This antibody binds the same epitope as clone FB11 and has a higher antigen-binding bivalent avidity than internally binding antiOAg mAb. This antibody is capable of detecting F. tularensis SchuS4 strain in an ELISA. It is also capable of agglutinating F. tularensis SchuS4 and live vaccine strain. |
Antibody First Published | Lu et al. The binding sites of monoclonal antibodies to the non-reducing end of Francisella tularensis O-antigen accommodate mainly the terminal saccharide. Immunology. (2013); 140(3)：374-89. PMID：23844703 |
Note on publication | Describes the generation, characterization and in vivo efficacy of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
