Key features and details | |
Cat. No. | MABL-1629 |
Name | Anti-Ft OAg mAbs |
Clone No. | AFD- N203 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Francisella tularensis, Francisella tularensis subsp. Holarctica |
Basic Information | |
Specificity | This antibody binds the internal O-antigen (OAg) of the gram-negative bacterium Francisella tularensis (Ft). |
Alternative Name | O-antigen of Francisella tularensis; O-antigen ligase family protein |
UniProt | A0A0B6E216 |
Immunogen | The original antibody was generated by intradermal immunization of BALB/cJ mice with sub- lethal dose of Francisella tularensis holarctica strain LVS followed by intraperitoneal booster immunization with an outer membrane- or capsule-enriched LVS preparation. |
Application Notes | This antibody is recommended for the detection of the O anitgen of F. tularensis through ELISA. It is reported to react with high molecular weight F. tularensis lipopolysaccharide chains in a western bloy assay. In a mouse model of respiratory tularemia with the highly virulent Ft type A strain SchuS4, this antibody reduced blood bacterial burden with potencies that mirror its antigen-binding strength. N203 prolonged survival in a dose dependent manner. This clone is a less potent binder than Ab52 (PMID: 25171003). |
Antibody First Published | Lu et al. Functional and structural characterization of Francisella tularensis O-antigen antibodies at the low end of antigen reactivity. Monoclon Antib Immunodiagn Immunother. (2014); 33(4)：235-45. PMID：25171003 |
Note on publication | Describes the generation and characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
