Key features and details | |
Cat. No. | MABL-1581 |
Name | Anti-FGFR1 mAbs |
Clone No. | AFD- C8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, WB, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for FGFR1. |
Alternative Name | CD331; FLT-2; BFGFR; bFGF-R-1; FGFR-1; N-sam; EU927378; EC:2.7.10.1;Fibroblast growth factor receptor 1; Basic fibroblast growth factor receptor 1; Fms-like tyrosine kinase 2; Proto-oncogene c-Fgr |
UniProt | P11362 |
Immunogen | The original antibody was isolated from a naïve library by phage display by panning against FGFR1. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected FGFR1 by western blot analysis. The antibody recognized the FGFR1-GFP construct in immunofluorescence analysis on HeLa cells. The binding affinity of the original format of the antibody to FGFR1 was measured by surface plasmon resonance (Kd= 9.3 nM). The antibody was successfully employed in a sandwich ELISA (Monega et al., 2009; PMID: 19196718). |
Antibody First Published | Monegal et al. Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library Protein Eng Des Sel. 2009 Apr;22(4):273-80. doi: 10.1093/protein/gzp002. Epub 2009 Feb 4. PMID:19196718 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
