Key features and details | |
Cat. No. | MABL-1552 |
Name | Anti-Factor VIII mAbs |
Clone No. | AFD- KM33 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, inhibit, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the C1 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A. |
Alternative Name | AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component |
UniProt | P00451 |
Immunogen | The original antibody was isolated from a human phage display library generated from the peripheral blood of an inhibitor patient and panning the library against factor VIII light chain. |
Application Notes | The scFv version of this antibody was capable of inhibiting factor VIII activity with inhibition titre of 97 BU/mg. The scFv antibody binds human factor VIII light chain with a binding affinity of Kd= 0.1 nM. It was reported that scFv KM33 inhibits the procoagulant activity of factor VIII, although its epitope is located outside region R1803-K1818 (PMID: 11159524). It was reported that the presence of this antibody completely inhibited FVIII endocytosis by both monocyte and bone marrow derived mouse dendritic cells (PMID: 21962992). It was reported that this antibody inhibits FVIII activity and interactions with its physiological ligands, von Willebrand factor, phospholipids and catabolic receptors in vitro (PMID: 11159524; Limburg V et al., 2005). |
Antibody First Published | van den Brink et al. Multiple VH genes are used to assemble human antibodies directed toward the A3-C1 domains of factor VIII. Blood. 2001 Feb 15;97(4):966-72. PMID:11159524 |
Note on publication | Describes the generation and epitope mapping of antibodies against A3 domain of factor VIII. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
