Key features and details | |
Cat. No. | MABL-1550 |
Name | Anti-Factor VIII mAbs |
Clone No. | AFD- EL-16 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, inhibit |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the C2 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A. |
Alternative Name | AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component; IT2 |
UniProt | P00451 |
Immunogen | The original antibody was isolated from a human phage display library generated from the peripheral blood of an inhibitor patient and panning the library against factor VIII light chain. |
Application Notes | The inhibitory activity of this antibody against factor VIII was determined using a one- stage clotting assay. This antibody was capable of completely eliminating FVIII inhibition by CLB-CAg 117, a C2 domain directed anti-factor VIII antibody (PMID: 10627462). |
Antibody First Published | van den Brink et al. Human antibodies with specificity for the C2 domain of factor VIII are derived from VH1 germline genes Blood. 2000 Jan 15;95(2):558-63. PMID:10627462 |
Note on publication | Describes the generation and epitope mapping of antibodies against C2 domain of factor VIII. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
