Anti-F1 mAbs

Cat. No.

MABL-1537

Name

Anti-F1 mAbs

Clone No.

AFD- Af1 8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC

Species Reactivity

Yersinia pestis

Basic Information

Specificity

This antibody is specific for the F1 capsule   protein from Yersinia pestis. It shows some cross- reaction with other Yersinia species that have a F1 protein. F1 is a protein that is part of the   capsule of Yersinia pestis. Yersinia pestis is the bacteria that causes the   plague in humans.

Alternative Name

F1 capsule antigen; caf1

UniProt

P26948

Immunogen

This antibody was generated by panning a   phage library prepared from 40 different samples of human peripheral blood   lymphocytes against the F1 protein.

Application Notes

To determine the ability of the scFv version   of this antibody to bind the F1 protein, an ELISA was performed on F1 using  the scFv version of this antibody, it showed strong binding. Furthermore, an   ELISA was also performed on Yersinia pestis to assess the binding in vivo.   Additionally, flow cytometry was performed on both Yersinia pestis and the F1   antigen, it showed a positive reaction to both (Lillo et al, 2011;   pmid:22174746).

Antibody First   Published

Lillo et al. Development of Phage-Based   Single Chain Fv Antibody Reagents for Detection of Yersinia pestis. PLoS One.  2011; 6(12): e27756. PMID:22174746

Note on publication

Phage antibody panning was performed against F1 in an automated fashion using the Kingfisher magnetic bead system. Selected scFvs were screened for F1-binding specificity by one-step alkaline phosphatase enzyme linked immunosorbant assay (ELISA), and assayed for binding to recombinant antigen and/or Y. pestis by flow cytometry and whole-cell ELISA.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.