Key features and details | |
Cat. No. | MABL-1535 |
Name | Anti-F protein mAbs |
Clone No. | AFD-2E 3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralization, SPR, ELISA |
Species Reactivity | RSV |
Basic Information | |
Specificity | The original antibody is specific for prefusion F protein. The antibody binds the protein through a conformational epitope spanning across three distinct sections of the RSV F protein: residues 419–434, 443–448, and 459–467. F-protein plays a pivotal role in the pathogenesis of RSV by mediating the fusion between the viral and the host cell membrane. F protein exists in two distinct conformations: the metastable prefusion conformation and the stable post fusion conformation. |
Alternative Name | fusion glycoprotein; Fusion glycoprotein F0 |
UniProt | P03420 |
Immunogen | A synthetic, fully human antibody library was used for panning against RSV prefusion and post fusion F proteins. The original antibody against RSV F protein was discovered following phage library panning and characterized. |
Application Notes | The specificity of the Fab and IgG1 format of the antibody was confirmed by ELISA analysis (EC50 value for IgG1 at 9.6 ng/mL). Surface plasmon resonance based studies demonstrated that the antibody bound exclusively to the prefusion conformation of the RSV F protein with an affinity Kd = 13 nM for monovalent Fab. The bivalent Fab exhibited neutralizing activities against both RSV A (Long) and B (Washington) strains with IC50 at 53.4 ng/mL and 515 ng/mL, respectively. The human IgG1 format showed an improved neutralizing activity against the RSV A strain (IC50 at 11.4 ng/mL) and comparable against the RSV B (Washington) strain (IC50 at 520 ng/mL). |
Antibody First Published | Chen et al. Discovery and Characterization of Phage Display-Derived Human Monoclonal Antibodies against RSV F Glycoprotein PLoS One. 2016; 11(6): e0156798. PMID:27258388 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
